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KMID : 0903520050480010053
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2005 Volume.48 No. 1 p.53 ~ p.59
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Alterations of Glutathione Peroxidase Patterns by Stressor Treatment in Rice Seedling Roots
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Kim Yoon-Kyoung
Lee Mi-Young
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Abstract
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The effect of various stressors such as reductant ascorbic acid, signalling molecules (salicylic acid and methyl jasmonic acid), heavy metals (NiCl2, and MnSO4) and NaCl on the glutathione peroxidase (GPX) activities and isoenzyme expression patterns were investigated in rice seedling roots. Total GPX activity increased according to the increase of ascorbic acid concentration. Prominent enhancement of GPX1 isozyme due to ascorbic acid contributed to the increase of total GPX activity. GPX showed different reactivity toward salicylic acid and methyl jasmonic acid. GPX activity increased at 0.1mM salicylic acid, and then decreased thereafter. However, GPX increased gradually in a methyl jasmonic acid concentration-dependent manner, and 3 fold increase of GPX activity was found at 1mM methyl jasmonic acid. Moreover, GPX1 isozyme increased according to the increase of salicylic acid, while GPX1 isozyme decreased according to the increase of methyl jasmonic acid. When metal ions were treated, GPX activity increased considerably according to the increase of NiCl2 concentration, however, GPX activity increased about 2 fold at 0.5mM CuSO4, and then decreased. Enhancement of GPX1 isozyme contributed to the increase of total GPX activities in NiCl2-treated and MnSO4-treated rice seedlings. Total GPX activity increased 1.7 fold in response to 300 mM NaCl. Especially GPX2 isozyme showed gradual increase according to the increase of NaCl concentration.
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KEYWORD
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glutathione peroxidase, stressors, total activity, isoenzyme pattern
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